Structural characterisation of MscM and investigation of protein-lipid dynamics of MscS in MSP1E3D1 nanodisc

Cell membranes play significant role in cells for the regulation of entry and exit of molecules. The cell membrane is comprised of lipids and integral membrane proteins. Mechanosensitive ion (MS) channels are membrane proteins that sense mechanical force and produce electrochemical information. These channels are seen across all forms of life, playing a vital role in the survival of some organisms. Bacterial MS channels, particularly Escherichia coli MscS and MscL, are extensively studied for the structure, function, and kinetics of these channels. MscM of the MscS-like family has an unresolved structure. This thesis covers a novel approach at resolving the structure of MscM. The approach is a curation of state-of-art techniques and aims to observe MscM in different conditions.