Murtaza, Mariyam (2012) The role of Crumbs2 in neural development. PhD thesis, University of Sheffield.
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The formation of a functionally integrated nervous system is dependent on a highly organized sequence of events that includes timely division and differentiation of progenitors. Three evolutionarily conserved polarity protein complexes are crucial for defining the apical and basolateral boundaries of cells. In my thesis, I demonstrate that one of the vertebrate homologs of Crumbs- Crumbs2 (Crb2) plays context dependent roles in the developing nervous system using two model systems: the chick embryonic hindbrain and the mouse embryonic telencephalon. In the developing telencephalon, conditional ablation of Crb2 leads to defects in recruitment of apical polarity proteins, cell junction proteins, positioning of mitotic cells and cortical neurogenesis. In the chick embryonic hindbrain, misexpression of Crb2 affects morphology of the neural tube and also affects the apical localization of cell polarity proteins, mitotic cell divisions and neural differentiation. In addition to this, I demonstrate that a novel secreted splice variant of Crb2 plays an important role in regulating neural crest cell migration. Taken together my analyses show that both loss and misexpression of Crb2 have similar effects on the apical domain and in confining mitotic cell divisions to the apical domain. This implies that the level of Crb2 is crucial for its various biological roles in the developing nervous system.
|Item Type:||Thesis (PhD)|
|Academic Units:||The University of Sheffield > Faculty of Science (Sheffield) > Biomedical Science (Sheffield)
The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > Biomedical Science (Sheffield)
|Depositing User:||Ms Mariyam Murtaza|
|Date Deposited:||11 Apr 2012 15:05|
|Last Modified:||08 Aug 2013 08:48|