The Development and Validation of a Quantitative Mass Spectrometric Method for the Analysis of Polypeptides in Human Plasma

Abstract

An assay for the quantitative analysis of the nonapeptide arginine-8-vasopressin has been developed. This utilised sample preparation techniques such as solid phase extraction in conjunction with ultra-high performance liquid chromatography. Methods to improve the limit of detection of AVP have been performed, utilising mass spectrometry. High field asymmetric ion mobility has been trialed to assess if the interface gave benefits. The limit of detection could not reach that of immunoassay techniques of 1 pg/mL but has been validated from 0.25-10 ng/mL.

Metadata

Supervisors:	Thomas-Oates, Jane and Turpin, Phillip
Awarding institution:	University of York
Academic Units:	The University of York > Chemistry (York)
Depositing User:	Miss Julie C Vincent
Date Deposited:	16 Mar 2011 12:50
Last Modified:	08 Aug 2013 08:46
Open Archives Initiative ID (OAI ID):	oai:etheses.whiterose.ac.uk:1353

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The Development and Validation of a Quantitative Mass Spectrometric Method for the Analysis of Polypeptides in Human Plasma

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