Rueangkham, Naruemon (2020) Modelling Cargo Transport and Deformation by Molecular Motors along Cytoskeletal Filaments. PhD thesis, University of Sheffield.
Abstract
In this thesis, we present a study of cargo transport or behaviors in a cell by clusters of molecular motors on a single and many parallel or anti-parallel mi- crotubules. Molecular motors can be processive motors or non-processive motors which can bind on and o� and switch between microtubules (lanes). Our study in- cludes analytical theory, simulations and a detailed comparison with experiments. We extend the analytic expressions for the non-processive motors to the case of �nite binding sites on a single lane and multiple lanes, as presented in chapter of one-lane and many-lane model, respectively. We performed Monte Carlo latticed based stochastic simulations to validate the corresponding system. The simula- tions also include simple exclusion process and sequence preservation of motors. Our results show that the limiting number of binding sites along the lanes and se- quence preservation reduce the probability of non-processive motors binding, but has a relatively small e�ect on the velocity. Multiple-lane transport can enhance the velocity and averaged run length and reduce the delivery time. We also study cargo behaviour using a tug-of-war model in which the lanes on which motors step are oriented in anti-parallel arrays between right and left ends of the cargo, as pre- sented in chapter of cargo deformation. This mechanism generates motor pulling force onto the cargo which results in changing its shape and position. The cargo can be any cargoes acting as a spring and the nucleus is the example of cargo that we are interested in. This model provides new insights of nuclear deformation and displacement caused by the motor pulling force. Our simulations show that the deformation and displacement can be induced by the increasing number of mi- crotubules. This corresponds with the experimental results of Susana Godinho's group.
Metadata
Supervisors: | Hawkins, Rhoda |
---|---|
Related URLs: | |
Keywords: | Molecular motor proteins, microtubules, cellular transporst |
Awarding institution: | University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Physics and Astronomy (Sheffield) |
Depositing User: | Miss Naruemon Rueangkham |
Date Deposited: | 12 Oct 2020 16:05 |
Last Modified: | 12 Oct 2020 16:05 |
Downloads
Final eThesis - complete (pdf)
Embargoed until: 12 October 2023
This file cannot be downloaded or requested.
Filename: Rueangkham160124363final.pdf

Supplementary Material
Embargoed until: 12 October 2023
This file cannot be downloaded or requested.
Filename: Extended embargo N Rueangkham (Science).pdf
Description: extended embargo form

Related datasets
Statistics
You can contact us about this thesis. If you need to make a general enquiry, please see the Contact us page.