White Rose University Consortium logo
University of Leeds logo University of Sheffield logo York University logo

Automated indentation analysis technique for the investigation of plant and fungal cell nano-mechanics using Atomic Force Microscopy

Sovatzoglou, Spyridon (2019) Automated indentation analysis technique for the investigation of plant and fungal cell nano-mechanics using Atomic Force Microscopy. PhD thesis, University of Sheffield.

[img] Text (PhD thesis)
Thesis_Sovatzoglou_final.pdf
Restricted until February 2021.

Request a copy

Abstract

In this project, we investigated the mechanics of different types of cells with the help of an Atomic force microscope (AFM) in force mapping mode. We investigated the stomatal mechanics of Arabidopsis Thaliana and its mutant pme6, and we also investigated the cell mechanics of the pathogenic yeast Candida albicans and one of its mutants missing the AP-2 adaptor complex. The pme6 mutant alters the pectin concentration in the cell wall and has an effect in both plant growth and the stomatal biological function, while the AP-2 mutation in C. albicans is considered to have an effect to cell wall’s regulation and its virulence. We developed a dynamic programming technique for the analysis of AFM force mapping experiments, and we implemented an analytical equation/Contact mechanics model found in literature for the calculation of the elastic modulus. We also discuss on the different modulus values resulting from the different models, and its relation to powel law exponent beta.

Item Type: Thesis (PhD)
Academic Units: The University of Sheffield > Faculty of Science (Sheffield) > Physics and Astronomy (Sheffield)
Depositing User: Dr Spyridon Sovatzoglou
Date Deposited: 23 Mar 2020 10:51
Last Modified: 23 Mar 2020 10:51
URI: http://etheses.whiterose.ac.uk/id/eprint/26283

Please use the 'Request a copy' link(s) above to request this thesis. This will be sent directly to someone who may authorise access.
You can contact us about this thesis. If you need to make a general enquiry, please see the Contact us page.

Actions (repository staff only: login required)