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Investigating the role of Capicua in mediating FGF transcriptional regulation in X. tropicalis

King, Michael Gavin (2018) Investigating the role of Capicua in mediating FGF transcriptional regulation in X. tropicalis. PhD thesis, University of York.

[img] Text (PhD Thesis)
Michael King Thesis 24-05-19.pdf - Examined Thesis (PDF)
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[img] Other (CIC-L human mouse alignment)
CIC-LH~1.GB - Supplementary Material
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[img] Other (CIC-L human mouse alignment pdf)
CIC-LH~1.PDF - Supplementary Material
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[img] Other (CIC-S human mouse alignment)
CIC-SH~1.GB - Supplementary Material
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[img] Other (CIC-S human mouse alignment pdf)
CIC-SH~1.PDF - Supplementary Material
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[img] Image (CIC alignments without exon 21)
CIC alignments without exon 21.bmp - Supplementary Material
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[img] Image (pCS2+ X. tropicalis CIC-S Map)
pCS2+ X. tropicalis CIC-S Map.png - Supplementary Material
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[img] Other (pCS2+ X. tropicalis CIC-S Map)
pCS2+ X. tropicalis CIC-S.dna - Supplementary Material
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[img] Other (Xenopus_tropicalis.capicua_annotation.2018-09-27 RNAseq)
Xenopus_tropicalis.capicua_annotation.2018-09-27.xlsx - Supplementary Material
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[img] Video (1. CIC-L targeting morpholino injections.)
1CIC-L~1.MPG - Supplementary Material
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[img] Video (2. CIC-S targeting morpholino injections.)
2CIC-S~1.MPG - Supplementary Material
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[img] Video (3. CIC-L & CIC-S targeting morpholino injections.)
3CIC-L~1.MPG - Supplementary Material
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[img] Video (4. Uninjected control.)
4UNINJ~1.MPG - Supplementary Material
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[img] Other (X. trop cDNA sequence CIC-L)
X. trop CIC-L cDNA.dna - Supplementary Material
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[img] Other (X. trop cDNA sequence CIC-S)
X. trop CIC-S cDNA.dna - Supplementary Material
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[img] Other (SnapGene Softwre)
snapgene_viewer_4.2.11_win.exe - Supplementary Material
Available under License Creative Commons Attribution-Noncommercial-No Derivative Works 2.0 UK: England & Wales.

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[img] Other (X. trop CIC gene Map)
X. trop CIC gene Map.dna - Supplementary Material
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Abstract

The Fibroblast Growth Factor (FGF) family of secreted peptides signal through tyrosine kinase receptors (RTKs), leading to the downstream activation of the MAP kinase (MAPK), causing transcriptional change. FGF signalling plays multiple critical roles in the maintenance of mesoderm induction, regulation of differentiation and patterning throughout early embryonic development. Despite extensive research on the FGF dependent transcriptome, there are still gaps in are understanding of gene regulation downstream of FGF signalling. The aim of this project was to address the gaps in knowledge by investigating a novel regulator of transcription, Capicua (CIC). D. melanogaster research has revealed that CIC functions as a transcriptional repressor downstream of RTKs, EGFR and Torso, regulated by Ras-MAPK signal transduction. This project’s focus was on establishing if CIC is involved as a transcriptional repressor downstream of FGF signalling, functioning in a similar fashion to EGFR and Torso gene transcription during early amphibian development. The work in this thesis has established the gene structure of CIC in X. tropicalis, allowing the analysis of temporal and spatial expression profiles of the prominent isoforms of CIC. Analysis has shown that CIC-L is expressed in the maternal phase of embryonic development, whilst CIC-S is zygotically expressed. Given the deep conservation of developmental mechanisms within vertebrate species and the increasing evidence linking both FGF signalling and the function of CIC in human health and disease, the output of this investigation will have a wide significance.

Item Type: Thesis (PhD)
Academic Units: The University of York > Biology (York)
Depositing User: Mr Michael Gavin King
Date Deposited: 04 Jun 2019 13:39
Last Modified: 04 Jun 2019 13:39
URI: http://etheses.whiterose.ac.uk/id/eprint/24117

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