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Heterologous Expression and Characterisation of Unspecific Peroxygenases

Mielke, Tamara (2016) Heterologous Expression and Characterisation of Unspecific Peroxygenases. PhD thesis, University of York.

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Abstract

In 2004, unspecific peroxygenases (UPOs) from fungi were first identified and showed to catalyse selective oxygenation reactions, with high turnovers, good stability and a broad reaction scope, providing a valuable alternative to established biocatalytic hydroxylation systems such as cytochrome P450s. At the start of this project, access to UPOs was limited to expression in their native fungi, hence studies were performed looking into heterologous expression of the enzymes from Agrocybe aegerita and Agaricus bisporus, followed by characterisation, and application of the enzymes.

Item Type: Thesis (PhD)
Academic Units: The University of York > Chemistry (York)
Depositing User: Ms Tamara Mielke
Date Deposited: 04 May 2018 16:09
Last Modified: 04 May 2018 16:09
URI: http://etheses.whiterose.ac.uk/id/eprint/20015

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