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Characterisation of cell walls at the feeding site of Meloidogyne incognita

Bozbuga, Refik (2017) Characterisation of cell walls at the feeding site of Meloidogyne incognita. PhD thesis, University of Leeds.

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Abstract

Meloidogyne incognita induces a unique feeding structure, termed giant cells, by reprogramming plant cells in the feeding site within host plant roots. The nematode modifies the function of cells including giant cell wall composition. Characterisation of pectin, hemicellulose and glycoproteins of giant cell walls formed in different hosts was analysed. In addition the role of cell-wall genes in nematode feeding site development was also analysed. In situ analysis was performed to determine the presence and distribution of giant cell wall components; cell wall elements were quantified using enzyme linked immunosorbent assay in nematode feeding sites on different host plants Arabidopsis thaliana (Brassicaceae), Vigna angularis (Fabaceae) and Zea mays (Poaceae). The cell wall compositions in the M. incognita feeding site were observed by comparison with uninfected root tissues. There were distinct responses in terms of detection of cell wall polysaccharides in the nematode feeding site of the different hosts. Modifications of cell wall polysaccharides in giant cells formed in Arabidopsis were minor compared to uninfected sections. By contrast, analysis of the giant cell wall formed in Vigna angularis revealed decreased amounts of mannan, xylan, galactan, processed arabinan, arabinogalactan protein and extensin. In Zea mays, xyloglucan, methyl esterified pectic homogalacturonan, galactan, arabinogalactan proteins increased in abundance in giant cell walls. Arabidopsis plants that carried mutations in cell-wall related genes were analysed. Mutants for genes important in the formation of hemicellulose (GLZ1, MSR1 and MUR3) together with those of the pectin related genes BGAL5 and RGXT1 all resulted in smaller gall development together with a concomitant reduction in nematode size in addition to a reduction in the number of nematodes recovered. Converselymutation of the pectin-related genes ARAD1 and ARAD2 or the glycoprotein-related genes led to increased susceptibility to the nematodes.

Item Type: Thesis (PhD)
Academic Units: The University of Leeds > Faculty of Biological Sciences (Leeds)
The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds)
Identification Number/EthosID: uk.bl.ethos.713250
Depositing User: Refik Bozbuga
Date Deposited: 15 May 2017 09:52
Last Modified: 25 Jul 2018 09:54
URI: http://etheses.whiterose.ac.uk/id/eprint/17049

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