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Leveraging the Power of Mass Spectrometry-Based Proteomics to Reveal Novel Biological Insights

Dowle, Adam Ashley (2016) Leveraging the Power of Mass Spectrometry-Based Proteomics to Reveal Novel Biological Insights. PhD thesis, University of York.

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Mass spectrometry-based proteomics has the potential to offer new qualitative and quantitative insights into a wide array of biological questions. The work presented in this thesis, including the associated submitted papers, evidences my contribution to this field. Paper 1 demonstrates the successful identification of post translational modifications imparted upon histones following DNA damage. Papers 2-4 employ a label-free quantification approach to study the immunomodulation molecules endemic parasites use to impair host resistance. Paper 5 uses an isobaric tagging approach to quantify changes in immune response when dendritic cells are exposed to medically important antigens. Paper 6 displays the power of mass spectrometry in identifying a novel co-factor, which could not be identified by X-ray crystallography. In paper 7 mass spectrometry is used to show that the remaining unidentified enzyme in the morphinan biosynthetic pathway is expressed as a fusion protein composed of two distinct enzymes. These studies are all linked in their reliance on my use of mass spectrometry-based proteomics to make original contributions to knowledge and understanding within their fields.

Item Type: Thesis (PhD)
Related URLs:
Keywords: Mass spectrometry. Proteomics.
Academic Units: The University of York > Chemistry (York)
Identification Number/EthosID: uk.bl.ethos.701469
Depositing User: Mr Adam A Dowle
Date Deposited: 13 Jan 2017 10:45
Last Modified: 24 Jul 2018 15:21
URI: http://etheses.whiterose.ac.uk/id/eprint/15722

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