White Rose University Consortium logo
University of Leeds logo University of Sheffield logo York University logo

Structural and functional studies of a Lytic Polysaccharide Monooxygenase

Gregory, Rebecca Charlotte (2016) Structural and functional studies of a Lytic Polysaccharide Monooxygenase. PhD thesis, University of York.

This is the latest version of this item.

RCGregory_2016_PhD.pdf - Examined Thesis (PDF)
Available under License Creative Commons Attribution-Noncommercial-No Derivative Works 2.0 UK: England & Wales.

Download (5Mb) | Preview


The enzymatic degradation of polysaccharides is a major goal of the biotechnology industry, most notably for both first (starch) and second (cellulose/chitin) generation biofuels. The classical model for enzymatic polysaccharide breakdown involves the action of endo- and exo- acting glycoside hydrolases which act in synergy; endo-acting enzymes generating free chain-ends for the processive exo-glycosidases. In 2010 a new enzyme class was discovered which overturned the classical hydrolase model. These enzymes, now known as Lytic Polysaccharide Monooxygenases (LPMOs), are understood to be copper-dependent oxygenase enzymes that cause chain cleavage within polysaccharides, facilitating their degradation by classical hydrolases. This research showcases the findings for an LPMO of the “Auxiliary Activity” class AA10 from the bacterium Bacillus amyloliquefaciens (BaAA10). Methods of gene expression and protein production are described, including the incorporation of a SUMO-tag which resulted in increased yields of BaAA10. The nature of the copper binding was assessed using Isothermal Titration Calorimetry and Differential Scanning Fluorimetry, which demonstrated the extremely tight binding of copper to the enzyme and its increased stability when copper is bound. Additionally, the detection of lactone and aldonic acid products of oxidative degradation, in MALDI-TOF Mass Spectrometry results, determined for the first time that BaAA10 was active on both alpha and beta forms of chitin. Finally, a Cu(II) structure of BaAA10 was able to be obtained following the use of a spiral data collection technique as a method of preventing photoreduction of Cu(II) to Cu(I) in the X-ray beam. This structure can now provide us with details regarding the copper active site and, together with potential spectroscopic studies in the future, can help us to determine the mechanism of action of these LPMO enzymes, so that we can better exploit their use within the biofuel industry.

Item Type: Thesis (PhD)
Related URLs:
Academic Units: The University of York > Chemistry (York)
Depositing User: Miss Rebecca Charlotte Gregory
Date Deposited: 29 Nov 2016 12:22
Last Modified: 29 Nov 2016 12:22
URI: http://etheses.whiterose.ac.uk/id/eprint/15659

Available Versions of this Item

  • Structural and functional studies of a Lytic Polysaccharide Monooxygenase. (deposited 29 Nov 2016 12:22) [Currently Displayed]

You do not need to contact us to get a copy of this thesis. Please use the 'Download' link(s) above to get a copy.
You can contact us about this thesis. If you need to make a general enquiry, please see the Contact us page.

Actions (repository staff only: login required)