Investigating afferent mechanisms involved in bladder hypersensitivity

This thesis investigates the afferent mechanism that may be involved in bladder hypersensitivity. The Study primarily focuses on the role of TRPM8 on bladder sensory firing. One part of the study also aims to replicate the ice water test in an in vitro mouse model to assess the contribution of TRPM8. An interesting aspect that the study investigates is the interaction between TRPM8 and TRPV1, TRPA1 and purinergic signalling. Finally, the study investigates ERβ KO mice as a model for interstitial cystitis. Investigating these parameters may reveal more information of the sensory changes that may occur in relation to bladder hypersensitivity, hence revealing novel targets for therapy.