Process and Formulation Optimization of a Continuous Microfluidic-Based Platform for High-Quality mRNA-LNP Production

Abstract

Messenger RNA (mRNA) has emerged as a transformative platform for
vaccines and targeted therapies due to its capacity for precise and rapid gene
expression. The COVID-19 pandemic underscored both its promise and the
challenges of large-scale production. In response, continuous flow
manufacturing—guided by Quality by Design (QbD) principles and enabled by
automation and digital technologies—offers a scalable, consistent, and efficient
alternative to traditional batch production.

Metadata

Supervisors:	Kis, Zoltan
Keywords:	mRNA-Lipid Nanoparticle, Continuous mRNA-LNP manufacturing, Quality by Design (QbD) process optimization, Real-time size-PDI monitoring
Awarding institution:	University of Sheffield
Academic Units:	The University of Sheffield > Faculty of Engineering (Sheffield) > Chemical and Biological Engineering (Sheffield)
Date Deposited:	01 Dec 2025 09:48
Last Modified:	01 Dec 2025 09:48
Open Archives Initiative ID (OAI ID):	oai:etheses.whiterose.ac.uk:37676

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Process and Formulation Optimization of a Continuous Microfluidic-Based Platform for High-Quality mRNA-LNP Production

Abstract

Metadata

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Final eThesis - complete (pdf)

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