Microscale mobile domains in supported lipid membranes for a more dynamic and accessible model system

This thesis presents an optimized method for generating large, mobile lipid domains in supported lipid bilayers (SLBs), visible under standard fluorescence microscopy. By systematically studying the effects of ionic conditions such as PBS and Mg²⁺, hydrodynamic decoupling of domains was achieved, enhancing their mobility. Photo-oxidation effects on unsaturated lipids were characterized and harnessed to manipulate membrane composition and phase behaviour. Advanced imaging techniques like FLIM and a custom domain-tracking tool enabled detailed analysis of domain dynamics. This work provides a robust model system for studying membrane biophysics and protein-lipid interactions.