Initial characterisation of MiCatS1 in human cells

The S-phase checkpoint ensures that mitosis is postponed when DNA replication fails to complete (Smythe & Newport, 1992). Bowen (2014) performed a whole-genome siRNA screen in HCT116 human colorectal carcinoma cells to identify additional genes with a role in S-phase checkpoint control. In addition to many previously identified checkpoint components, four genes were identified which increase the frequency with which cells undergo mitosis in the absence of prior DNA replication, resulting in mitotic catastrophe. These genes are referred to as MiCatS (Mitotic Catastrophe in S phase) 1-4. We have begun a molecular characterisation of MiCatS1 in HCT116 cells.