Oligo number,Target/associated gene,Sequence,Direction,Notes,,,, OL5674,Blasticidin resistance gene,GACGATACAAGTCAGGTTG,Reverse,Blasticidin resistance cassette for diagnostic PCRs 1,,,, OL6137,gDNA,aaaagcaccgactcggtgccactttttcaagttgataacggactagccttattttaacttgctatttctagctctaaaac,Reverse,Standard primer for amplification of sgDNA,,,, OL6365,LmxM.36.6340,ACCCCCCCTCCCCTGAGCATCGCAGGTGATgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6366,LmxM.36.6340,CCAATCATCGGCATTGTTGCCGGAGCTCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6367,LmxM.36.6340,gaaattaatacgactcactataggACCATTGCTCTCAAAACAAGgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6368,LmxM.29.0910,GGTCGACGTCGGCTTGCCTCGCAGCTCCCTgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6369,LmxM.29.0910,ACTGCTGCCGTTGAACACAAATTGGCTCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6370,LmxM.29.0910,gaaattaatacgactcactataggAGTCCTTCGCACAAACACTGgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6371,LmxM.07.0280,CCCCCTCTCTCTGTTTCTCGGCGAGACCCTgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6372,LmxM.07.0280,GAACAGGTGGACAAGGAGCTGCGGGTCCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6373,LmxM.07.0280,gaaattaatacgactcactataggAAGGGTTCCAATAGTTGGGGgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6374,LmxM.13.1470,CCTCGTCCGGCAACACACACACATACAGACgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6375,LmxM.13.1470,GCGGACGTAGAGGGAGCTAAGTTGGTCCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6376,LmxM.13.1470,gaaattaatacgactcactataggACACACACACGCCAATAAAAgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6377,LmxM.36.4370,AACGCTGATATAGAGCCCACCACTCTCTTCgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6378,LmxM.36.4370,GGTCAGCAATACTAACGAGCCCATCGCCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6379,LmxM.36.4370,gaaattaatacgactcactataggTCCATTACACGGCAGCACTGgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6380,LmxM.26.2370,CGTGACCAGCTCAGGCACCTAGGGCAGCGGgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6381,LmxM.26.2370,TGCCGCTGAGGGCTGAGATGGCGACTCCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6382,LmxM.26.2370,gaaattaatacgactcactataggAGGACGCAACCAGAGGCAAAgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6383,LmxM.31.1090,TGGAGCACACCTCAGCCACGGTACATTCCGgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6384,LmxM.31.1090,CCCCTCCATGTAAAAGCGAAAGCCGTCCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6385,LmxM.31.1090,gaaattaatacgactcactataggACTAAGGTGAGGACGTCACAgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6386,LmxM.31.3930,GATCGTCCCGCGTGTCGCGGTCGTTGGTGAgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6387,LmxM.31.3930,GATCCAATTGTCGATCCCTGCGAAGCTCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6388,LmxM.31.3930,gaaattaatacgactcactataggCTTTGGTGCGGTTTCCATCTgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6389,LmxM.33.3960,GTGATTGCTCTCGTTGTGGGGCCGACTTATgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6390,LmxM.33.3960,CCTCGCTGACTCGTCTCTCCGCGAGGTCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6391,LmxM.33.3960,gaaattaatacgactcactataggTTCACAAAGTACTCTGAGGCgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6392,LmxM.34.2450,CCTTCAACCCTCCTCCACCCTTCCCTCCCTgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6393,LmxM.34.2450,CGCCTTTGGATCATAGACGCCGACGTCCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6394,LmxM.34.2450,gaaattaatacgactcactataggTACCAAGAGCGAGTACTGTGgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6395,LmxM.34.5390,AGAAAGATAGTTATCATCACGCGGCGGTGCgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6396,LmxM.34.5390,CTCTGAGAAGTCGGAGCTGTGCAACTCCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6397,LmxM.34.5390,gaaattaatacgactcactataggTAACAATAGCGTTTCTACGGgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6398,LmxM.29.2170,GACACTGACGCGGCCGCGAATATATCTATTgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6399,LmxM.29.2170,GTACTCAGTGCCGTACCCAGGATCATACATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6400,LmxM.29.2170,gaaattaatacgactcactataggGCCATTACGACATGGGAAAAgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6836,LmxM.02.0390,CGCTTACAGGACGCAGGGTGTGTGGGGAACgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6837,LmxM.02.0390,GCGCGACTGCGCCAGTGACAGCCCTGACATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6838,LmxM.02.0390,gaaattaatacgactcactataggACCCTCTCTGTCGCTCCCAAgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6839,LmxM.04.0680,CGTTGTACGCCTCCCTTCGCTCCCCGTCCTgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6840,LmxM.04.0680,CGTCTCCTTGATAATGCGCGTTGTAAGCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6841,LmxM.04.0680,gaaattaatacgactcactataggAGAGTGGGGAAGGGTGGATAgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6842,LmxM.05.0930,TCCCCGTGTGTCTTTGGACTCCTTCTGCCCgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6843,LmxM.05.0930,TCGCTTGATGGAGGAAGACGCGTACGACATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6844,LmxM.05.0930,gaaattaatacgactcactataggGAGAACAGGGGTGGGGGTGGgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6845,LmxM.07.0850,AAGTCAGGGGACCGACTCTCCCCTGTGCCGgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6846,LmxM.07.0850,CTTACCGAGCCGCACAAGTGGATTGGGCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6847,LmxM.07.0850,gaaattaatacgactcactataggTGCGTGTGTGTATGTGTGTAgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6848,LmxM.13.1580,ATCTCTCCTCCCTCGGTGGTCTTTTCCCCAgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6849,LmxM.13.1580,CAGGCGAAAGTTTCGCGGCACCTCGACCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6850,LmxM.13.1580,gaaattaatacgactcactataggGTGCAAGTGAATATGTTGTCgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, Ol6851,LmxM.22.0610,CTTTTTTAATTGGTGTGAGCGCCGTCTTTCgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6852,LmxM.22.0610,AATCCGAGTCGGGAGACGAGCTGCCTGCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6853,LmxM.22.0610,gaaattaatacgactcactataggCTCTTGCTTCCTTCACAAGCgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6854,LmxM.24.1710,TTTTTCTCCTCGCTCACTTCATTTCAGATCgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6855,LmxM.24.1710,ACACACACACACACACACACACAAATGCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6856,LmxM.24.1710,gaaattaatacgactcactataggTGTACAGTGTGTCTTCAAATgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6857,LmxM.24.2130,CGTGCCTGCTCTTGTGCGCTTGCGTCGGTGgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6858,LmxM.24.2130,TGCCCCGCTGTTGTTGCCGCCGCCGTGCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6859,LmxM.24.2130,gaaattaatacgactcactataggCAACGCGCTTCTCTGGTCTTgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6860,LmxM.31.0700,TCTTCTCCGTTGCTGTTTCTTGCTCCACCAgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6861,LmxM.31.0700,GTTGCTGCGGAGGTTGCCTGCGCCGCTCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6862,LmxM.31.0700,gaaattaatacgactcactataggGTGGTGGCGGCTGGGATCTGgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6863,LmxM.31.0960,AACGGAAGAAGAATTAAAAGGCGCCCTCCTgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6864,LmxM.31.0960,CTCCTTCTGAATGTGCGGAAGAGCCGACATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6865,LmxM.31.0960,gaaattaatacgactcactataggTGATGATGATGATGCCACTTgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6866,LmxM.32.2770,GCACAGACACACATACACACACACACAGACgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6867,LmxM.32.2770,CGTCCGCATGCCAGCCGCCGTCAGAGGCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6868,LmxM.32.2770,gaaattaatacgactcactataggCTTGTAGCGAGGAGTAGGAAgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6869,LmxM.33.0900,ACCAAAGAGTTCCAGTCCAGACCTTCCCCAgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6870,LmxM.33.0900,TTTCAGGCATTGCGCGGTAGGGGCCGTCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6871,LmxM.33.0900,gaaattaatacgactcactataggACAGGTGGGAAGGCGAGTTAgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6872,LmxM.33.1555,TCCTTCATGTTTGCTGGGGTTTTTTTTTCAgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6873,LmxM.33.1555,GTTCGCTCGCCGCACAGTGCGCACTGACATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6874,LmxM.33.1555,gaaattaatacgactcactataggCGACGGCTCGTGTCGTTTTTgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6875,LmxM.34.1300,CCTATCTGTTCTGTTGCTCTCATCTCACCCgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6876,LmxM.34.1300,CAGCTCCTTCTGGATCCGGCGAAGCGCCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6877,LmxM.34.1300,gaaattaatacgactcactataggTGTTGTTTTTCTTTCCTTTCgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6878,LmxM.24.2140,ATCACGTCAACCCTAACCGCATCCCCACCGgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6879,LmxM.24.2140,CCCGTAGGGCGAGCAGGATGAGGACGCCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6880,LmxM.24.2140,gaaattaatacgactcactataggGTCGATGAGGATGATACACGgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6881,LmxM.23.0550,TGCTGGCACTGGCGCACGTCAAGGTAACCAgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6882,LmxM.23.0550,CTGCCGTTTTTGCTCCTCCTCGGACAGCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6883,LmxM.23.0550,gaaattaatacgactcactataggCGCGCCTGAGTGTGAGTGCGgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6884,LmxM.34.3060,CAGACACGAAAACATAGCTTCAAACTACCGgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6885,LmxM.34.3060,GTCGATCAACACTTCCTTCTCAGCCTCCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6886,LmxM.34.3060,gaaattaatacgactcactataggTTGTGCTTTGCGCTGTGTTTgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6887,LmxM.08.0220,TGTGGTGCGCCAAGAACGCCCACACGCCCAgtataatgcagacctgctgc,Forward,Upstream forward primer for tagging,,,, OL6888,LmxM.08.0220,TACGACAGAGCCGATACAGCCGTCGCTCATactacccgatcctgatccag,Reverse,Upstream reverse primer for tagging,,,, OL6889,LmxM.08.0220,gaaattaatacgactcactataggCTACGCTACTCGTTACTTTGgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL6890,LmxM.01.0710,GTGCAGTGGCGAGGCCCGCCTGTGCGGGCGggttctggtagtggttccgg,Forward,Upstream forward primer for tagging,,,, OL6891,LmxM.01.0710,TGACCAGCGGTAGAACCTCCGTTCGCCCCGccaatttgagagacctgtgc,Reverse,Upstream reverse primer for tagging,,,, OL6892,LmxM.01.0710,gaaattaatacgactcactataggACGACGGCGTAGATGCGCGAgttttagagctagaaatagc,Forward,5' sgRNA primer,,,, OL7153,LmxM.36.6340,ACCCCCCCTCCCCTGAGCATCGCAGGTGATTAATACGACTCACTATAAAACTGGAAGAAATTAACCCTTgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL7154,LmxM.36.6340,GAAGGGGAGGCGGCGCAAACGAAACGACCCccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL7155,LmxM.36.6340,gaaattaatacgactcactataggTAGCAGGGCGTATCGAACGTgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL7156,LmxM.29.0910,GGTCGACGTCGGCTTGCCTCGCAGCTCCCTTAATACGACTCACTATAAAACTGGAAGTTGCATGTGGCCgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL7157,LmxM.29.0910,CTCGTTCGTACTGCTCCACGAAGCCCACCGccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL7158,LmxM.29.0910,gaaattaatacgactcactataggCGTGGCGCTACAACGCGTCTgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL7159,LmxM.07.0280,CCCCCTCTCTCTGTTTCTCGGCGAGACCCTTAATACGACTCACTATAAAACTGGAAGCCCGGAACCCTTgtataatgcagacctgctgc,Forward,Upstream forward primer 1 for knockout,,,, OL7160,LmxM.07.0280,CGCACACGCCTCTGCCCCCTCCCCCTCCCCccaatttgagagacctgtgc,Reverse,Downstream reverse primer 1 for knockout,,,, OL7161,LmxM.07.0280,gaaattaatacgactcactataggAGTAGTAGAGTGGGGGGAGGgttttagagctagaaatagc,Reverse,3' sgRNA primer 1,,,, OL7162,LmxM.13.1470,CCTCGTCCGGCAACACACACACATACAGACTAATACGACTCACTATAAAACTGGAAGAACGTTGTGTCCgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL7163,LmxM.13.1470,GAGCAAACATGCACCCCCTCCCCATCCCCTccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL7164,LmxM.13.1470,gaaattaatacgactcactataggATCAGCTGCCACGTATGTGGgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL7165,LmxM.36.4370,AACGCTGATATAGAGCCCACCACTCTCTTCTAATACGACTCACTATAAAACTGGAAGGGGCCAACCCTTgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL7166,LmxM.36.4370,CCCCTCCAACAAGCAAGCATCCCACGCCCGccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL7167,LmxM.36.4370,gaaattaatacgactcactataggGCACGTGGAGCTCTTTGACGgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL7168,LmxM.26.2370,CGTGACCAGCTCAGGCACCTAGGGCAGCGGTAATACGACTCACTATAAAACTGGAAGCACACCATGTCCgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL7169,LmxM.26.2370,CACGTCAGGAGCAACGCTGCATGGGCTCCGccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL7170,LmxM.26.2370,gaaattaatacgactcactataggTCACCGCCGTAGACGGAAAGgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL7171,LmxM.31.1090,TGGAGCACACCTCAGCCACGGTACATTCCGTAATACGACTCACTATAAAACTGGAAGTTTGGTTCCCGGgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL7172,LmxM.31.1090,ACTCCGCAACAAACGTCTATACGGTCACATccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL7173,LmxM.31.1090,gaaattaatacgactcactataggATAGCTGCTTTGACAGCCTGgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL7174,LmxM.31.3930,GATCGTCCCGCGTGTCGCGGTCGTTGGTGATAATACGACTCACTATAAAACTGGAAGGTGTGCATGTCCgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL7175,LmxM.31.3930,CTTCGCAGATACGTACGCATAGAAAGACGCccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL7176,LmxM.31.3930,gaaattaatacgactcactataggGTCGGTGCACATGCTCGAGAgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL7177,LmxM.33.3960,GTGATTGCTCTCGTTGTGGGGCCGACTTATTAATACGACTCACTATAAAACTGGAAGCCCAATTCCCGGgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL7178,LmxM.33.3960,GTGAGCCCTTCGCAACGGCGGGGCGCGGCTccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL7179,LmxM.33.3960,gaaattaatacgactcactataggAAACAACCAAGCATGCCGCGgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL7180,LmxM.34.2450,CCTTCAACCCTCCTCCACCCTTCCCTCCCTTAATACGACTCACTATAAAACTGGAAGTGTGTCATGTCCgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL7181,LmxM.34.2450,GATGCAACAGGCATAAATACAAAAGAGAAAccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL7182,LmxM.34.2450,gaaattaatacgactcactataggCGAAAAGAGCGCGAGATGAAgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL7183,LmxM.34.5390,AGAAAGATAGTTATCATCACGCGGCGGTGCTAATACGACTCACTATAAAACTGGAAGAAACCTTCCCGGgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL7184,LmxM.34.5390,CAGATGAGCAACAGCACCAAAAGAAAAGGCccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL7185,LmxM.34.5390,gaaattaatacgactcactataggGACAACGAAAGGAATGACGCgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL7186,LmxM.29.2170,GACACTGACGCGGCCGCGAATATATCTATTTAATACGACTCACTATAAAACTGGAAGATGACGTTGTCCgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL7187,LmxM.29.2170,CCTCTCTCCCCGTTTCCTGTACTGCTGCCAccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL7188,LmxM.29.2170,gaaattaatacgactcactataggTTCCTACCAGTCAGTGCTGTgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL7994,LmxM.36.6340,AGTTTGGGTGGTTTCCGTGT,Forward,Within gene forward (checking knockouts),,,, OL7995,LmxM.36.6340,GTATTTGCCGCGTTCTCGAC,Reverse,Within gene reverse (checking knockouts),,,, OL7996,LmxM.36.6340,GAGTTTCTCGCGTCACCTCA,Forward,Upstream of HR region (checking knockouts),,,, OL7997,LmxM.29.0910,CCTCCTTGCCCACCAAAGAT,Forward,Within gene forward (checking knockouts),,,, OL7998,LmxM.29.0910,GAGCGCACATACATTGCCAG,Reverse,Within gene reverse (checking knockouts),,,, OL7999,LmxM.29.0910,TCTTTCGCTTTCAGGCTGTCT,Forward,Upstream of HR region (checking knockouts),,,, OL8001,LmxM.07.0280,AGCTGCAGAAGGTCAAGTCC,Forward,Within gene forward (checking knockouts),,,, OL8002,LmxM.07.0280,CTTACCGTTCGGCTCCAACT,Reverse,Within gene reverse (checking knockouts),,,, OL8003,LmxM.07.0280,AGGGTGGGTACATCAGTGCT,Forward,Upstream of HR region (checking knockouts),,,, OL8004,LmxM.13.1470,CAACGCTGCAACAGCTTCTT,Forward,Within gene forward (checking knockouts),,,, OL8005,LmxM.13.1470,AGTCACGACTGATCTCCCGA,Reverse,Within gene reverse (checking knockouts),,,, OL8006,LmxM.13.1470,GGGCGCGCACTCATTTTTAT,Forward,Upstream of HR region (checking knockouts),,,, OL8007,LmxM.36.4370,TCCTCGTTGGACTCAAGCAC,Forward,Within gene forward (checking knockouts),,,, OL8008,LmxM.36.4370,GATCGGCGAACGATCTGACT,Reverse,Within gene reverse (checking knockouts),,,, OL8009,LmxM.36.4370,GCCACTCTCGTGGTTACTCA,Forward,Upstream of HR region (checking knockouts),,,, OL8011,LmxM.26.2370,CGTAATGGCACTCAATGGCG,Forward,Within gene forward (checking knockouts),,,, OL8012,LmxM.26.2370,GATACATGGCTCCCTCGTCG,Reverse,Within gene reverse (checking knockouts),,,, OL8013,LmxM.26.2370,CGGTCTCTACGCGATTCGTT,Forward,Upstream of HR region (checking knockouts),,,, OL8349,LmxM.31.1090,ACCTTCGAGACGTTCCCAAC,Forward,Within gene forward (checking knockouts),,,, OL8350,LmxM.31.1090,CGCTGGTTGCAGTAAGGAGA,Reverse,Within gene reverse (checking knockouts),,,, OL8351,LmxM.31.1090,AACTGCTTCCGTCTGACGTT,Forward,Upstream of HR region (checking knockouts),,,, OL8353,LmxM.31.3930,TGACGACAACGGTGGAAGAG,Forward,Within gene forward (checking knockouts),,,, OL8354,LmxM.31.3930,CCAAGCGCTTCGTAATGCTC,Reverse,Within gene reverse (checking knockouts),,,, OL8355,LmxM.31.3930,TCACGTTGGTGGCGTTTTTC,Forward,Upstream of HR region (checking knockouts),,,, OL8356,LmxM.33.3960,CAAGGTGAGGACCTGCTGAG,Forward,Within gene forward (checking knockouts),,,, OL8357,LmxM.33.3960,AGAACAGACTGCCCGTAAGC,Reverse,Within gene reverse (checking knockouts),,,, OL8358,LmxM.33.3960,GGGATCCGGAGAAACAACCA,Forward,Upstream of HR region (checking knockouts),,,, OL8360,LmxM.34.2450,GATCTAGAGCGGTGGTTCCG,Forward,Within gene forward (checking knockouts),,,, OL8361,LmxM.34.2450,TACCGCATGAGGTGCGATAC,Reverse,Within gene reverse (checking knockouts),,,, OL8362,LmxM.34.2450,TGGAGGGCGAGAGAAGAACT,Forward,Upstream of HR region (checking knockouts),,,, OL8364,LmxM.34.5390,CACGGTTGTGAAGGGCAAAG,Forward,Within gene forward (checking knockouts),,,, OL8365,LmxM.34.5390,CAATGATTTCGCAGGAGGCG,Reverse,Within gene reverse (checking knockouts),,,, OL8366,LmxM.34.5390,TCGTTCATCCTTCGGTGCAA,Forward,Upstream of HR region (checking knockouts),,,, OL8367,LmxM.29.2170,TCGCAGTGGAACGCTACTTT,Forward,Within gene forward (checking knockouts),,,, OL8368,LmxM.29.2170,GACCTGTTGCACAGGAGACA,Reverse,Within gene reverse (checking knockouts),,,, OL8369,LmxM.29.2170,GCGATCTTTACTGCCAACGC,Forward,Upstream of HR region (checking knockouts),,,, OL8640,LmxM.32.2770,GCACAGACACACATACACACACACACAGACTAATACGACTCACTATAAAACTGGAAGGCAATGCGCTGGgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL8641,LmxM.32.2770,TACATCATCTCAGCAGACAGACATAACCAAccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL8642,LmxM.32.2770,gaaattaatacgactcactataggAACCTGGAACCAAGTGCGTTgttttagagctagaaatagc,Reverse,3' sgRNA primer for knockout,,,, OL8643,LmxM.24.2140,ATCACGTCAACCCTAACCGCATCCCCACCGTAATACGACTCACTATAAAACTGGAAGATTTCCTAGCAAgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL8644,LmxM.24.2140,GACGCAACAACAAGAATACATCGACCTCCTccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL8645,LmxM.24.2140,gaaattaatacgactcactataggGCGCCGGCGATGATGACAAGgttttagagctagaaatagc,Reverse,3' sgRNA primer for knockout,,,, OL8684,Cassette barcode,TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGAgatgatgattacTAATACGACTCACTATAAAACTGGAAG,Forward,Nextera adaptor+variable region+target specific CRISPR knockout integration,,,, OL8685,Cassette barcode,GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGAGAGACAGGCATGCCTT,Reverse,Nextera adaptor+target specific CRISPR knockout integration,,,, OL9327,Blasticidin resistance gene,CACAAGGTCCCCCAGTAAAA,Reverse,Blasticidin resistance cassette for diagnostic PCRs 2,,,, OL9371,LmxM.23.0550,TGCTGGCACTGGCGCACGTCAAGGTAACCATAATACGACTCACTATAAAACTGGAAGGGCTTCCGGTGGgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL9372,LmxM.23.0550,CAAATCACCATTAGTCATCCTCTGTGGCCGccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL9373,LmxM.23.0550,gaaattaatacgactcactataggCCGCGTGGTGTGGCCATCTAgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL9374,LmxM.34.3060 ,CAGACACGAAAACATAGCTTCAAACTACCGTAATACGACTCACTATAAAACTGGAAGCCTCAGTACCAAgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL9375,LmxM.34.3060 ,CCGGGTGGGGTGGGCATGGCCGGGGGGCGTccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL9376,LmxM.34.3060,gaaattaatacgactcactataggGCAGCCAGCAGCTCCGGATTgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL9377,LmxM.08.0220,TGTGGTGCGCCAAGAACGCCCACACGCCCATAATACGACTCACTATAAAACTGGAAGTTAGGCCGGTGGgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL9378,LmxM.08.0220,TGATTCATGGCAAGTCGTCACCGCGGTCCAccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL9379,LmxM.08.0220 ,gaaattaatacgactcactataggGTGCTTGCGTCTGGAGCACGgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL9380,LmxM.01.0710,GTTTGAGCAACAAGCACCGGAGCAGTGCCTTAATACGACTCACTATAAAACTGGAAGAAGACGTACCAAgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL9381,LmxM.01.0710,TGACCAGCGGTAGAACCTCCGTTCGCCCCGccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL9382,LmxM.01.0710 ,gaaattaatacgactcactataggACGACGGCGTAGATGCGCGAgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL9383,LmxM.02.0390,CGCTTACAGGACGCAGGGTGTGTGGGGAACTAATACGACTCACTATAAAACTGGAAGGCTATATCGTGGgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL9384,LmxM.02.0390,TCCCCAAGCCCCACACGCACACAAAAGCCTccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL9385,LmxM.02.0390,gaaattaatacgactcactataggACGCGAGAGAAAGGTTGCGAgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL9386,LmxM.04.0680 ,CGTTGTACGCCTCCCTTCGCTCCCCGTCCTTAATACGACTCACTATAAAACTGGAAGATATCTCTCCAAgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL9387,LmxM.04.0680,TGCACACGCACGCACGCACATGTCAGGCATccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL9388,LmxM.04.0680 ,gaaattaatacgactcactataggAGGCATACACACATGTCACAgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL9389,LmxM.05.0930 ,TCCCCGTGTGTCTTTGGACTCCTTCTGCCCTAATACGACTCACTATAAAACTGGAAGCGATAATCGTGGgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL9390,LmxM.05.0930 ,TACGTCGCGGCGCTACCGCCACTCCCCCCCccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL9391,LmxM.05.0930,gaaattaatacgactcactataggTGTGTGTGTGTGTGTGTGGAgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL9392,LmxM.07.0850 ,AAGTCAGGGGACCGACTCTCCCCTGTGCCGTAATACGACTCACTATAAAACTGGAAGTATAGTCTCCAAgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL9393,LmxM.07.0850,GCAGTCGCCCACCCCCACCCCCCACTGCCAccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL9394,LmxM.07.0850,gaaattaatacgactcactataggCTGGAGAGGAACCAAAACGAgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL9395,LmxM.13.1580,ATCTCTCCTCCCTCGGTGGTCTTTTCCCCATAATACGACTCACTATAAAACTGGAAGTAGCGATCGTGGgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL9396,LmxM.13.1580 ,AAAGCACCGAGACAATCAACAGTGGCTCCAccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL9397,LmxM.13.1580 ,gaaattaatacgactcactataggTTTCGCAGTATCACACCCGCgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL9398,LmxM.22.0610 ,CTTTTTTAATTGGTGTGAGCGCCGTCTTTCTAATACGACTCACTATAAAACTGGAAGCGCGATCTCCAAgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL9399,LmxM.22.0610,AACCAGGGAACTAGCCACGCACATGCCGACccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL9400,LmxM.22.0610 ,gaaattaatacgactcactataggACACCGCACCTGTAGCAGTGgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL9401,LmxM.24.1710 ,TTTTTCTCCTCGCTCACTTCATTTCAGATCTAATACGACTCACTATAAAACTGGAAGATCGCATCGTGGgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL9402,LmxM.24.1710 ,ACGACACGGCGAGGGCCCGCCAAGTCGTGCccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL9403,LmxM.24.1710 ,gaaattaatacgactcactataggGGTGGAGCGCGTGAAGGCGGgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL9404,LmxM.24.2130,CGTGCCTGCTCTTGTGCGCTTGCGTCGGTGTAATACGACTCACTATAAAACTGGAAGGCGCTTCTCCAAgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL9405,LmxM.24.2130 ,ATCTCTAGGACCCAACAAACCACCGACTCAccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL9406,LmxM.24.2130,gaaattaatacgactcactataggGTCACACACGCAACACGTAGgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL9407,LmxM.31.0700 ,TCTTCTCCGTTGCTGTTTCTTGCTCCACCATAATACGACTCACTATAAAACTGGAAGTGAATTACGCGGgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL9408,LmxM.31.0700,ACACCTTCACGAGGAATGCCTCAAGCGCACccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL9409,LmxM.31.0700,gaaattaatacgactcactataggGAGTAAGCGGTGGGTATAAGgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL9410,LmxM.31.0960,AACGGAAGAAGAATTAAAAGGCGCCCTCCTTAATACGACTCACTATAAAACTGGAAGCATTCAGTCTAAgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL9411,LmxM.31.0960,TCGTACGTCGCGCTGTTTTGGCTGCTACCGccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL9412,LmxM.31.0960,gaaattaatacgactcactataggTCGACGTCACGGCCAGACCTgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL9413,LmxM.33.0900,ACCAAAGAGTTCCAGTCCAGACCTTCCCCATAATACGACTCACTATAAAACTGGAAGGGAAGAGGACGGgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL9414,LmxM.33.0900,CTTCGTAGTTTATCAGAGGGAGGTTCGCCGccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL9415,LmxM.33.0900 ,gaaattaatacgactcactataggAGTGAACACAGATCCCTAGGgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL9416,LmxM.33.155,TCCTTCATGTTTGCTGGGGTTTTTTTTTCATAATACGACTCACTATAAAACTGGAAGACGGCGCATTCCgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL9417,LmxM.33.1555 ,TTGACACGAAGTGGTTGCACAAGCGAGTATccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL9418,LmxM.33.1555,gaaattaatacgactcactataggGACCGGGTACTAGTTCCGTAgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL9419,LmxM.34.1300 ,CCTATCTGTTCTGTTGCTCTCATCTCACCCTAATACGACTCACTATAAAACTGGAAGCCTTCAGGAGGGgtataatgcagacctgctgc,Forward,Upstream forward primer for knockout,,,, OL9420,LmxM.34.1300 ,TCTTCGGTTCTCTCCTTGGTTTCACTTCCCccaatttgagagacctgtgc,Reverse,Downstream reverse primer for knockout,,,, OL9421,LmxM.34.1300,gaaattaatacgactcactataggAAAGTGAATCCGGGAGCGGTgttttagagctagaaatagc,Reverse,3' sgRNA primer,,,, OL9479,LmxM.32.2770,CCAAAGGGTTTCTTCCGCAC,Forward,Within gene forward (checking knockouts),,,, OL9480,LmxM.32.2770,TGTTTGCCATGGACGATGCT,Reverse,Within gene reverse (checking knockouts),,,, OL9481,LmxM.32.2770,CATTTCGGCACACGCTTTCA,Forward,Upstream of HR region (checking knockouts),,,, OL9482,LmxM.24.2140,GCATGGATTCTGCGTTGACC,Forward,Within gene forward (checking knockouts),,,, OL9483,LmxM.24.2140,GGTACAAAGACGCGCTTCAC,Reverse,Within gene reverse (checking knockouts),,,, OL9484,LmxM.24.2140,AATGTACAAGCGCACCCACT,Forward,Upstream of HR region (checking knockouts),,,, OL9545,LmxM.23.0550,ATTCTGGAGCGGTGCAAAGA,Forward,Within gene forward (checking knockouts),,,, OL9546,LmxM.23.0550,ACGAGAGACGTGGTTGTCAC,Reverse,Within gene reverse (checking knockouts),,,, OL9547,LmxM.23.0550,GAGGTCGGCGAATGAAAGGT,Forward,Upstream of HR region (checking knockouts),,,, OL9548,LmxM.34.3060,GACTTTATCGCTGCGGCAAG,Forward,Within gene forward (checking knockouts),,,, OL9549,LmxM.34.3060,CCGTTCCACAGCATACCCTT,Reverse,Within gene reverse (checking knockouts),,,, OL9550,LmxM.34.3060,TTTCATCTCGTACCCGCCTG,Forward,Upstream of HR region (checking knockouts),,,, OL9551,LmxM.01.0710 ,CACCGTCGTCGACATGGATT,Forward,Within gene forward (checking knockouts),,,, OL9552,LmxM.01.0710,TGGGCCATGTGAATGACTCG,Reverse,Within gene reverse (checking knockouts),,,, OL9553,LmxM.01.0710,CTGTCGACATCGCTCCTAGTC,Forward,Upstream of HR region (checking knockouts),,,, OL9554,LmxM.02.0390,TCCGTCTCTCCCTCAAGTACAA,Forward,Within gene forward (checking knockouts),,,, OL9555,LmxM.02.0390 ,TAGCCCTTCCAATGATGCCT,Reverse,Within gene reverse (checking knockouts),,,, OL9556,LmxM.02.0390,ATCGTGAGAGGGAGACGGG,Forward,Upstream of HR region 1 (checking knockouts),,,, OL9557,LmxM.04.0680,ACAACGCGCATTATCAAGGAG,Forward,Within gene forward (checking knockouts),,,, OL9558,LmxM.04.0680,GGCTCGACATGAGAATCTGGA,Reverse,Within gene reverse (checking knockouts),,,, OL9559,LmxM.04.0680,TGCCTCTTACCCATCCAACAC,Forward,Upstream of HR region (checking knockouts),,,, OL9560,LmxM.05.0930,ACGCGTCTTCCTCCATCAAG,Forward,Within gene forward (checking knockouts),,,, OL9561,LmxM.05.0930,GCCTCCTTTGTTTCGAGTGC,Reverse,Within gene reverse (checking knockouts),,,, OL9562,LmxM.05.0930 ,GACTCCGATCATCCCGTGTG,Forward,Upstream of HR region (checking knockouts),,,, OL9563,LmxM.07.0850,CCGACCCGGACATTCATCTG,Forward,Within gene forward (checking knockouts),,,, OL9564,LmxM.07.0850,AGACTCGCGTAACCCTCCAT,Reverse,Within gene reverse (checking knockouts),,,, OL9565,LmxM.07.0850,AATCGAGATCCGCTTACCGC,Forward,Upstream of HR region (checking knockouts),,,, OL9754,LmxM.07.0280,TCGGCACGCGCGCTTGCGCAGGAGTCGATGccaatttgagagacctgtgc,Reverse,Downstream reverse primer 2 for knockout,,,, OL9755,LmxM.07.0280,gaaattaatacgactcactataggGGGAAGGGTTCCAATAGTTGGGGgttttagagctagaaatagc,Forward,5' sgRNA primer 2,,,, OL9756,LmxM.07.0280,gaaattaatacgactcactataggTGGACACGCTGAGCGAGACCGGGgttttagagctagaaatagc,Reverse,3' sgRNA primer 2,,,, OL10120,LmxM.02.0390,ACGCGCACGAATTCTTTTCC,Forward,Upstream of HR region 2 (checking knockouts),,,,* Not sure which primer set I used but probably used the first batch. OL10121,LmxM.08.0220 ,TACCTGGGGCTCTCGATTCA,Forward,Within gene forward (checking knockouts),,,, OL10122,LmxM.08.0220,TTGAGTTCCCGACATCTGCC,Reverse,Within gene reverse (checking knockouts),,,, OL10123,LmxM.08.0220,GTACGCACCTCCCAACACTT,Forward,Upstream of HR region (checking knockouts),,,, OL10124,LmxM.13.1580,GAAACTTTCGCCTGCTGGA,Forward,Within gene forward (checking knockouts),,,, OL10125,LmxM.13.1580,TCAGTACGTAGACCCCTCCG,Reverse,Within gene reverse (checking knockouts),,,, OL10126,LmxM.13.1580,TAAGCGCAATTCCATCCCGT,Forward,Upstream of HR region (checking knockouts),,,, OL10127,LmxM.22.0610 ,GATCAACCTCAGCAGGGTGT,Forward,Within gene forward (checking knockouts),,,, OL10128,LmxM.22.0610,GCGCCTCTAGGTTAAGCGAT,Reverse,Within gene reverse (checking knockouts),,,, OL10129,LmxM.22.0610,CGTGAAGAGGCCGCAAAAAT,Forward,Upstream of HR region (checking knockouts),,,, OL10132,LmxM.24.1710,CTCCCTTCCTTCTGCCAGTG,Forward,Upstream of HR region (checking knockouts),,,, OL10166,LmxM.31.0700,TCCAGCTGCCATCCGATTAC,Forward,Within gene forward (checking knockouts),,,, OL10167,LmxM.31.0700,GTCCGTCAAACCCTTCGAGT,Reverse,Within gene reverse (checking knockouts),,,, OL10168,LmxM.31.0700,TCTGGCTGATACCGACACAC,Forward,Upstream of HR region (checking knockouts),,,, OL10169,LmxM.31.0960,TGGACGAGTGTGCATTTCCA,Forward,Within gene forward (checking knockouts),,,, OL10170,LmxM.31.0960,CGCCACGATGCTTGATTAGC,Reverse,Within gene reverse (checking knockouts),,,, OL10171,LmxM.31.0960,CGCAGACTTGGCATCGAGTA,Forward,Upstream of HR region (checking knockouts),,,, OL10172,LmxM.33.0900,AGCTCTTACCACCCCGAGAA,Forward,Within gene forward (checking knockouts),,,, OL10173,LmxM.33.0900,CGGGCTACAGCACCAGTAAT,Reverse,Within gene reverse (checking knockouts),,,, OL10174,LmxM.33.0900,GGAAGAGGTTGCGTCCTTG,Forward,Upstream of HR region (checking knockouts),,,, OL10175,LmxM.33.1555,TCTTAAGGAGCTGGCCAACAG,Forward,Within gene forward (checking knockouts),,,, OL10176,LmxM.33.1555,CTTGAACTCTTCAGGCGTTGC,Reverse,Within gene reverse (checking knockouts),,,, OL10177,LmxM.33.1555,TAGTCGCAGTTCGCAGAGTG,Forward,Upstream of HR region (checking knockouts),,,, OL10283,LmxM.24.2130,CGGCTGCAAAAGGAACTCGTA,Forward,Within gene forward (checking knockouts),,,, OL10284,LmxM.24.2130,GTACAGCGATGGTGAACTGCT,Reverse,Within gene reverse (checking knockouts),,,, OL10285,LmxM.24.2130,GATTCCTGCGGCTGTTCCTA,Forward,Upstream of HR region,,,, OL10329,LmxM.24.1710,CATGCAGAAGCTGGATGGAGT,Forward,Within gene forward (checking knockouts),,,, OL10330,LmxM.24.1710,GATGATGTAGTTGCCGCCCAT,Reverse,Within gene reverse (checking knockouts),,,, OL10540,Recoded LmxM.13.1580,TCCAGGGACCAGCAATGGTCGAGGTTCCGCGC,Forward,Forward primer for integration into pETFPP vectors,,,, OL10541,Recoded LmxM.13.1580,TGAGGAGAAGGCGCGTCAGTAGGTACTACCCTCC,Reverse,Reverse primer for integration into pETFPP vectors,,,, OL10602,pETFPP vectors,TAATACGACTCACTATAGGG,Forward,T7 sequencing primer,,,, OL10603,pETFPP vectors,TATGCTAGTTATTGCTCAGCGGT,Reverse,T7 term sequencing primer,,,, OL10633,pGL2813 (recoded UBA1a),CACGGTGACCTGTGTCGTAA,Forward,Sequencing primer,,,, OL10634,pGL2813 (recoded UBA1a),GTTCTTCGGCTGCCGTTTTT,Forward,Sequencing primer,,,, OL10635,pGL2813 (recoded UBA1a),ATGGGACCAGAAACGGAAGG,Forward,Sequencing primer,,,, OL11111,UBC2,CGCGAGTGGACGCGCAAGTACGCCAAGCCGggttctggtagtggttccgg,F,Primer for C-terminal tagging with mNeonGreen,,,, OL11112,UBC2,TGCACACGCACGCACGCACATGTCAGGCATccaatttgagagacctgtgc,R,Primer for C-terminal tagging with mNeonGreen,,,, OL11113,UBC2,gaaattaatacgactcactataggAGGCATACACACATGTCACAgttttagagctagaaatagc,Guide,Primer for C-terminal tagging with mNeonGreen,,,, OL11114,MPK3,AGCTCAAAGAACGGTAGCGGTCACCATCACggttctggtagtggttccgg,F,Primer for C-terminal tagging with mNeonGreen,,,, OL11115,MPK3,CCTGCGTGAGCGGGATGGCACACACAGTCGccaatttgagagacctgtgc,R,Primer for C-terminal tagging with mNeonGreen,,,, OL11116,MPK3,gaaattaatacgactcactataggAAACACAACACGACGGCGCCgttttagagctagaaatagc,Guide,Primer for C-terminal tagging with mNeonGreen,,,, OL11117,UEV1,CTGGTGCAGCCACCGGAGGGGTCTACGTACggttctggtagtggttccgg,F,Primer for C-terminal tagging with mNeonGreen,,,, OL11118,UEV1,AAAGCACCGAGACAATCAACAGTGGCTCCAccaatttgagagacctgtgc,R,Primer for C-terminal tagging with mNeonGreen,,,, OL11119,UEV1,gaaattaatacgactcactataggTTTCGCAGTATCACACCCGCgttttagagctagaaatagc,Guide,Primer for C-terminal tagging with mNeonGreen,,,, OL11120,MPK3,TCAGTCTCTTTTTTTATTTCCTGTTACCCGgtataatgcagacctgctgc,F,Forward primer for N-terminal tagging,,,, OL11122,MPK3,gaaattaatacgactcactataggACTGGCTGGTGTGGCTACTGgttttagagctagaaatagc,Guide,Guide for N-terminal tagging,,,, OL11148,MPK3,CACGCTCAGCTCCTGGTTGCTCTTGTGCATagaaccggaaccggaaccac,R,Reverse primer for N-terminal tagging with myc only,,,, OL11149,UBC2,CGTCTCCTTGATAATGCGCGTTGTAAGCATagaaccggaaccggaaccac,R,Reverse primer for N-terminal tagging with myc only,,,, OL11150,UEV1,CAGGCGAAAGTTTCGCGGCACCTCGACCATagaaccggaaccggaaccac,R,Reverse primer for N-terminal tagging with myc only,,,, OL11755,MPK3 N-terminal Myc tag,TCACTTTCACTACCGCCCTC,Forward,Checking tagged cell lines,,,, OL11756,MPK3 N-terminal Myc tag,CGTGTACTTGGACGGAACCT,Reverse,Checking tagged cell lines,,,, OL11759,UBC2 N-terminal Myc tag,TGCCGTCATCGACCAAGATA,Forward,Checking tagged cell lines,,,, OL11760,UBC2 N-terminal Myc tag,TGAAGTAGCGCGGGTTTTCC,Reverse,Checking tagged cell lines,,,, OL11763,UEV1 N-terminal Myc tag,TCGGTGGTCTTTTCCCCAGA,Forward,Checking tagged cell lines,,,, OL11764,UEV1 N-terminal Myc tag,TCGGGTAGTTCTCATCGCAG,Reverse,Checking tagged cell lines,,,, RB1,Recoded LmxM.23.0550,TCCAGGGACCAGCAATGCTTTCTGAGGAAGAGCAAAAAC,Forward ,Forward primer for integration into pETFPP vectors,,,, RB2,Recoded LmxM.23.0550,TGAGGAGAAGGCGCGTTAAAAGCGATAGCGGTAGCGGATG,Reverse,Reverse primer for integration into pETFPP vectors,,,, RB3,Recoded LmxM.04.0680,TCCAGGGACCAGCAATGTTGACCACTCGTATCATTAAGG,Forward ,Forward primer for integration into pETFPP vectors,,,, RB4,Recoded LmxM.04.0680,TGAGGAGAAGGCGCGTCATGGTTTGGCGTACTTACGAG,Reverse,Reverse primer for integration into pETFPP vectors,,,,