Oligo #,Oligo name,Sequence,Description
1203,"
1203.FWD_Tlg2_for_pSSU",ATCGATATGGCGACTCGTGACC,"Amplification of Tlg2 gene for cloning into 
pSSU vector"
1204,1204.REV_Tlg2_for_pSSU_3xHAtag,"
CCTAGGAGCGTAATCTGGAACATCGTATGGG
TAAGCGTAATCTGGAACATCGTATGGGTAAG
CGTAATCTGGAACATCGTATGGGTATCAGGT
AATGGCCTTGAAGA","Amplification of Tlg2 gene for cloning into 
pSSU vector"
1205,"
1205.FWD_Tlg2_3'UTR_for_pSSU",CCTAGGGGTGAGACACAGGGTGTGTG,"Amplification of tlg2 3'UTR for cloning into 
pSSU vector"
1206,"
1206.REV_Tlg2_3'UTR_for_pSSU",ACTAGTGCACTTCTCTGATGCAGCCA,"Amplification of tlg2 3'UTR for cloning into 
pSSU vector"
1207,"
1207.FWD_NMT_3'UTR_for_pSSU",CCTAGGTCTACGCACTGCCCCACCTT,"Amplification of nmt 3'UTR for cloning into 
pSSU vector"
1208,"
1208.REV_NMT_3'UTR_for_pSSU",ACTAGTGTGGTGAGCGGCTCAGCACT,"Amplification of nmt 3'UTR for cloning into 
pSSU vector"
1222,"
1222.Tlg2_KO_FWD_v2","TCCGTGCGCGTGTTCGGGCCGATGCTGCC
GGTATAATGCAGACCTGCTGC","
Amplication of resistance cassette to knockout Tlg2 using CRISPR/Cas9. Designed to amplify from pTBlast_v1 and pTPuro_v1 plasmids"
1223,1223.Tlg2_KO_REV_v2,"AAAAGACAGATCCGTGCGGCGCACACACCCC
CAATTTGAGAGACCTGTGC","
Amplication of resistance cassette to knockout Tlg2 using CRISPR/Cas9. Designed to amplify from pTBlast_v1 and pTPuro_v1 plasmids"
1224,"

1224.Tlg2_KO_5'sgRNA_v2",GAAATTAATACGACTCACTATAGGAGACACGTTGACCCAACTCGGTTTTAGAGCTAGAAATAGC,Amplication of 5'sgRNA for Tlg2 knockout
1225,"

1225.Tlg2_KO_3'sgRNA_v2","GAAATTAATACGACTCACTATAGGATTACCT
GAGGTGAGACACAGTTTTAGAGCTAGAAA
TAGC",Amplication of 3'sgRNA for Tlg2 knockout
984,"
984.Vps45_KO_FWD","CTCTAGTTGGTCGGCGCATGGAGTCAACCGG
TATAATGCAGACCTGCTGC","
Amplication of resistance cassette to knockout Vps45a using CRISPR/Cas9. Designed to amplify from pTBlast_v1 and pTPuro_v1 plasmids"
985,985.Vps45_KO_REV,"GCAGACTAGCTAGCGAGGCTACGGGAGCCCC
CAATTTGAGAGACCTGTGC","
Amplication of resistance cassette to knockout Vps45a using CRISPR/Cas9. Designed to amplify from pTBlast_v1 and pTPuro_v1 plasmids"
986,"
986.Vps45_KO_5'sgRNA","GAAATTAATACGACTCACTATAGGCAGCTACA
AAGTCGGAATGGGTTTTAGAGCTAGAAATAGC",Amplication of 5'sgRNA for Vps45 knockout
987,"987.Vps45_KO_3'sg
RNA","GAAATTAATACGACTCACTATAGGGTGCTGCAG
CTCGTTAGACTGTTTTAGAGCTAGAAATAGC",Amplication of 3'sgRNA for Vps45 knockout
,1143.E.P_OL6137_sgRNA_R,AAAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC,Common sgRNA primer for amplification
1197,"
1197.Tlg2KO_InGene
_Check_FWD",TGACGCCGCTTTGGGTGAAA,To determine presence or absence of gene in knockouts
1198,"
1198.Tlg2KO_InGene
_Check_REV",CCGCTCATCTGCCATCTGCT,To determine presence or absence of gene in knockouts
1199,"
1199.Tlg2KO_5'UTR
_Check_FWD",ACTGCCTGAGTTGTTGAGGCAAA,To check resistance cassette integration from 5'UTR of gene
1200,"
1200.Vps45KO_In
Gene_Check_FWD",CTAGACCGCAACGACGACCC,To determine presence or absence of gene in knockouts
1201,"
1201.Vps45KO_In
Gene_Check_REV",TTCGAGTTGACGCGGTCGAG,To determine presence or absence of gene in knockouts
1202,"
1202.Vps45KO_5'UTR
_Check_FWD",TCGTTGCTGCCCTGAGACAC,To check resistance cassette integration from 5'UTR of gene
1144,"

1144.E.P_OL9369",GCAGCAGGTCTGCATTATAC,To check resistance cassette integration (from within resistance cassette)
1249,"
1249.pSSU_polylinker
_FWD","CGATTTCGAAGAGCTCCCGGGGATCCACCGGT
GCTAGCCTGCAGGGTACCAGATCTA",Multiple cloning site for integretion into pSSU vector
1250,"
1250.pSSU_polylinker
_REV","CTAGTAGATCTGGTACCCTGCAGGCTAGCACC
GGTGGATCCCCGGGAGCTCTTCGAAAT",Multiple cloning site for integretion into pSSU vector
1331,"
1331.Tlg2_Blast_Cas
_Fwd","AAGCAGCGCCGTTACGTGCAAGTAGAAAGCGT
ATAATGCAGACCTGCTGC",Amplification of blasticidin S deaminase (BSD) gene for cloning into pSSU vector
1332,"
1332.Tlg2_Blast_Cas
_Rev","CCGTGCGGCGCACACACCCTGTGTCTCACCTAC
ACAGCTTGAGCAACGCG",Amplification of blasticidin S deaminase (BSD) gene for cloning into pSSU vector
1499,"
1499.Tlg2Npeptide
_FWD",GACGGTATCGATATGGCGACTCGTGAC,"To amplify Tlg2, for use for N-peptide "
1500,"
1500.Tlg2Npeptide_
REV","ATCTGGAACATCGTATGGGTAATTCATATTCTC
CTCTTC","To amplfy 34aa N-peptide for Tlg2, with overhang 
to match HA-tag"
1501,"1501.HaTag_Tlg2
Npeptide",CCTAGGTCATGCGTAATCGGGCACGTCGTACGGATATGCATAGTCTGGCACATCATAGGGATAAGCGTAATCTGGAACATCGTATGGGTAATTCATATTCTCCTCTTCGG,HA-tag with overhang to end of Tlg2 N-peptide 
1502,"
1502.Tlg2Npeptide
_fusionREV",CTCACCCCTAGGTCATGCGTAATCG,To amplify full Tlg2 N-peptide + HA-tag product 
1784,"
1784.Lmex_NMT_
qPCR_F",GCCAAAGACGGTGGCCGATA,For qPCRs
1785,"
1785.Lmex_NMT_
qPCR_R",GGCGTCCACCACTCAAATGT,For qPCRs
1786,"
1786.Lmex_Histone
H4_qPCR_F",TACTGAAGGCCTACGTGGAG,For qPCRs
1787,"
1787.Lmex_Histone
H4_qPCR_R",AGCGCATTCACAACATCGG,For qPCRs
1788,"
1788.Lmex_Sherp
_qPCR_F",AAGGGACCAGATGAGCAACG,For qPCRs
1789,"
1789.Lmex_Sherp
_qPCR_R",CACCATCCTTCAGCTCCTGG,For qPCRs
1790,"
1790.Lmex_Tlg2_
qPCR_F",CTCCTACACACGCGACCTC,For qPCRs
1791,"
1791.Lmex_Tlg2_
qPCR_R",GGCACATCTTGACATTGCGC,For qPCRs