Kershaw, Charles (2017) Raman Spectroscopy Studies of Prostate Cancer and Streptomyces Bacteria. MSc by research thesis, University of York.
Abstract
This thesis will examine the use of Raman spectroscopy (RS) to probe biomolecular information from biological samples. The research is comprised of two main studies, a prostate cancer study and a Streptomyces bacteria study. The prostate cancer study used live cell samples from two different patients (H517{15 and H554{15), containing normal and Gleason seven cancer cells and two standard cell lines, PNT2{C2 (normal) and P4E6 (Gleason four cancer). The Raman spectra of each cancer/normal matched pair were analysed using principle component analysis (PCA). PCA was able to distinguish the normal cells from the cancer cells for the H517{15 and standard cell line samples. However, the H554{15 cancer and normal cells comparison proved to be too similar for PCA to separate. Peak intensity ratio (PIR) analyses were used to probe biomolecular differences between the normal and cancer cell samples. PIR results for the H554{15 sample showed that the cancer and normal cell samples were similar. PIR results for H517{15 revealed several biomarkers, which are consistent with the biological literature on cancer. The PIR results for the standard cell lines suggested that the samples may not be representative of patient biology due to the PIR biomarkers showing opposite trends to both the H517{ 15 primary patient sample, and the literature. The second aim of this research was to assess changes in lipid content between three samples of Streptomyces bacteria. The three bacteria samples comprised the wild type (J1929), a strain which had undergone a mutation causing a loss of intrinsic antibiotic resistance (DT3017), and a treated form of the mutated strain (PDT16). The Raman study in this work corroborated known biological information about the samples, showing an increase in unsaturated fatty acid (lipid) content from the J1929 sample to the DT3017 sample, followed by a recovery to the J1929 lipid content, upon treatment, for the PDT16 sample.
Metadata
Supervisors: | Hancock, Yvette |
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Awarding institution: | University of York |
Academic Units: | The University of York > Physics (York) |
Depositing User: | Mr Charles Kershaw |
Date Deposited: | 07 Sep 2017 09:27 |
Last Modified: | 27 Jul 2018 00:18 |
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